Increased Transglycosylation Activity of Rhodotorula glutinis Endo-β-Glucanase in Media Containing Organic Solvent | Zendy

Tadao Oikawa | Zendy; Yasuyuki TSUKAGAWA | Zendy; Masashi CHINO | Zendy; Kenji Soda | Zendy

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Increased Transglycosylation Activity of Rhodotorula glutinis Endo-β-Glucanase in Media Containing Organic Solvent

Author(s) -

Tadao Oikawa,

Yasuyuki TSUKAGAWA,

Masashi CHINO,

Kenji Soda

Publication year - 2001

Publication title -

bioscience biotechnology and biochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.509

H-Index - 116

eISSN - 1347-6947

pISSN - 0916-8451

DOI - 10.1271/bbb.65.1889

Subject(s) - acetone , chemistry , hydrolysis , cellulase , rhodotorula , glucanase , solvent , organic solvent , yeast , chromatography , organic chemistry , enzyme , biochemistry , chemical engineering , engineering

The transglycosylation of p-nitrophenyl-beta-D-cellotrioside to cellotetraose catalyzed by endo-1,4-beta-glucanase (cellulase, EC 3.2.1.4) from a psychrotrophic yeast, Rhodotorula glutinis KUJ 2731, was increased by addition of a miscible organic solvent in the reaction mixture. Among various organic solvents tested, acetone was most effective. The transglycosylation activity increased with an increase in acetone concentrations, while hydrolysis activity was suppressed. The transglycosylation preferably occurred at acidic pH with the optimum pH at 2 in 10 mM Gly-HCl buffer. The optimum temperature of transglycosylation was found to be 50 degrees C in the presence of 40% acetone.

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