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Trehalose-producing Operon treYZ from Arthrobacter ramosus S34
Author(s) -
Takuo Yamamoto,
Kazuhiko Maruta,
Hikaru Watanabe,
Hiroshi Yamashita,
Michio Kubota,
Shigeharu Fukuda,
Masashi Kurimoto
Publication year - 2001
Publication title -
bioscience biotechnology and biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.509
H-Index - 116
eISSN - 1347-6947
pISSN - 0916-8451
DOI - 10.1271/bbb.65.1419
Subject(s) - trehalose , operon , arthrobacter , biochemistry , biology , chemistry , computational biology , gene , enzyme , escherichia coli
Arthrobacter ramosus S34, which produces trehalose from maltooligosaccharide, was isolated. A trehalose-producing operon, treYZ, was cloned from the genome. Expression experiments with treY and treZ confirmed that they coded malto-oligosyltrehalose synthase and malto-oligosyltrehalose trehalohydrolase, respectively. The amino acid sequence of TreY from A. ramosus S34 and that from Arthrobacter sp. Q36 did not show high identity, nor did those of TreZ.

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