Visualization of Biallelic Expression of the Imprinted SNRPN Gene Induced by Inhibitors of DNA Methylation and Histone Deacetylation | Zendy

Atsushi Kohda | Zendy; Hiroshi Taguchi | Zendy; Katsuzumi Okumura | Zendy

Open Access

Visualization of Biallelic Expression of the Imprinted SNRPN Gene Induced by Inhibitors of DNA Methylation and Histone Deacetylation

Author(s) -

Atsushi Kohda,

Hiroshi Taguchi,

Katsuzumi Okumura

Publication year - 2001

Publication title -

bioscience biotechnology and biochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.509

H-Index - 116

eISSN - 1347-6947

pISSN - 0916-8451

DOI - 10.1271/bbb.65.1236

Subject(s) - biology , dna methylation , microbiology and biotechnology , histone , epigenomics , transcription (linguistics) , gene expression , dna , cosmid , chromatin , methylation , genomic imprinting , genetics , gene , linguistics , philosophy

We have adapted a fluorescence in situ hybridization (FISH) method to detect nascent RNA molecules of the imprinted SNRPN gene at the initial transcription sites in nuclei of human HL60 and WI38 cells. Simultaneous detection of RNA and DNA of SNRPN by FISH using the cosmid probe confirmed its monoallelic expression in these cell lines. Treatment of both cells by inhibitors of DNA methylation and histone deacetylation resulted in time-dependent increase of the cell population with the biallelic expression of SNRPN.

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