Cloning of a Full-length cDNA Encodingent-Kaurene Synthase fromGibberella fujikuroi: Functional Analysis of a Bifunctional Diterpene Cyclase | Zendy

Tomonobu Toyomasu | Zendy; Hiroshi Kawaide | Zendy; Atsuko ISHIZAKI | Zendy; Shoko Shinoda | Zendy; Minoru Otsuka | Zendy; Wataru Mitsuhashi | Zendy; Takeshi Sassa | Zendy

Open Access

Cloning of a Full-length cDNA Encodingent-Kaurene Synthase fromGibberella fujikuroi: Functional Analysis of a Bifunctional Diterpene Cyclase

Author(s) -

Tomonobu Toyomasu,

Hiroshi Kawaide,

Atsuko ISHIZAKI,

Shoko Shinoda,

Minoru Otsuka,

Wataru Mitsuhashi,

Takeshi Sassa

Publication year - 2000

Publication title -

bioscience biotechnology and biochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.509

H-Index - 116

eISSN - 1347-6947

pISSN - 0916-8451

DOI - 10.1271/bbb.64.660

Subject(s) - complementary dna , gibberella fujikuroi , biology , diterpene , biochemistry , atp synthase , rapid amplification of cdna ends , microbiology and biotechnology , nucleic acid sequence , molecular cloning , gene , genetics , gibberellin

We report here the nucleotide sequence of a full-length cDNA encoding ent-kaurene synthase that was isolated by a reverse-transcription polymerase chain reaction from Gibberella fujikuroi (Gcps/ks). This cDNA encodes 952 amino acid residues with a relative molecular mass of 107 kDa. The sequence similarity between Gcps/ks and ent-kaurene synthase of the gibberellin A1-producing fungus, Phaeosphaeria sp. L487, is very high, suggesting that Gcps/ks is also a bifunctional diterpene cyclase. Its recombinant protein expressed in Escherichia coli converted geranylgeranyl diphosphate to copalyl diphosphate and ent-kaurene.

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