Production of Isopropylcis-6-Hexadecenoate by Regiospecific Desaturation of Isopropyl Palmitate by a Double Mutant of aRhodococcusStrain | Zendy

K Koike | Zendy; Mikio Takaiwa | Zendy; Katsutoshi Ara | Zendy; Shigeo Inoue | Zendy; Yoshiharu Kimura | Zendy; Susumu Ito | Zendy

Open Access

Production of Isopropylcis-6-Hexadecenoate by Regiospecific Desaturation of Isopropyl Palmitate by a Double Mutant of aRhodococcusStrain

Author(s) -

K Koike,

Mikio Takaiwa,

Katsutoshi Ara,

Shigeo Inoue,

Yoshiharu Kimura,

Susumu Ito

Publication year - 2000

Publication title -

bioscience biotechnology and biochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.509

H-Index - 116

eISSN - 1347-6947

pISSN - 0916-8451

DOI - 10.1271/bbb.64.399

Subject(s) - isopropyl , chemistry , bioconversion , strain (injury) , mutant , yield (engineering) , incubation , lecithin , stereochemistry , medicinal chemistry , biochemistry , fermentation , biology , materials science , gene , metallurgy , anatomy

Resting cells of a double mutant noted as KSM-MT66, derived from Rhodococcus sp. strain KSM-B-3 by UV irradiation, were found to cis-desaturate isopropyl hexadecanoate, yielding isopropyl cis-6-hexadecenoate. Addition of sodium glutamate (1.0%), Mg SO4 (2 mM), and thiamine (2 mM) increased the productivity of the unsaturated product in phosphate buffer. Optimal temperature and pH for the reaction were around 26 degrees C and 7, respectively. Under the optimized conditions, more than 50 g/l of isopropyl cis-6-hexadecenoate was produced after a 3-day incubation by resting cells of the mutant. Thus, cis-6-hexadecenoic acid, the main component of human sebaceous lipids, can be manufactured economically by the rhodococcal bioconversion.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research