Open AccessGene Encoding a Dextransucrase-like Protein inLeuconostoc mesenteroidesNRRL B-512F
Author(s) -
Kazumi Funane,
Kouichi Mizuno,
Hidenari Takahara,
Mikihiko Kobayashi
Publication year - 2000
Publication title -
bioscience, biotechnology, and biochemistry
Language(s) - Uncategorized
Resource type - Journals
SCImago Journal Rank - 0.509
H-Index - 116
eISSN - 1347-6947
pISSN - 0916-8451
DOI - 10.1271/bbb.64.29
Subject(s) - dextransucrase , leuconostoc mesenteroides , glucosyltransferase , gene , biochemistry , leuconostoc , microbiology and biotechnology , nucleic acid sequence , chemistry , sucrose , biology , bacteria , genetics , lactobacillus , fermentation , lactic acid
A gene, dsrT, encoding a dextransucrase-like protein was isolated from the genomic DNA libraries of Leuconostoc mesenteroides NRRL B-512F dextransucrase-like gene. The gene was similar to the intact open reading frames of the dextransucrase gene dsrS of L. mesenteroides NRRL B-512F, dextransucrase genes of strain NRRL B-1299 and streptococcal glucosyltransferase genes, but was truncated after the catalytic domain, apparently by the deletion of five nucleotides. dsrT mRNA was produced in this strain L. mesenteroides when cells were grown in a sucrose medum, but at a level of 20% of that of dsrS mRNA. The molecular weight of the dsrT gene product was 150,000 by SDS-PAGE. The product did not synthesize dextran, but had weak sucrose cleaving activity. The insertion of five nucleotides at the putative deletion point in dsrT resulted in an enzyme with a molecular weight of 210,000 and with dextransucrase activity.