Introduction of Enterostatin (VPDPR) and a Related Sequence into Soybean Proglycinin A1aB1bSubunit by Site-directed Mutagenesis | Zendy

Yasuyuki Takenaka | Zendy; Shigeru Utsumi | Zendy; Masaaki Yoshikawa | Zendy

Open Access

Introduction of Enterostatin (VPDPR) and a Related Sequence into Soybean Proglycinin A_1aB_1bSubunit by Site-directed Mutagenesis

Author(s) -

Yasuyuki Takenaka,

Shigeru Utsumi,

Masaaki Yoshikawa

Publication year - 2000

Publication title -

bioscience biotechnology and biochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.509

H-Index - 116

eISSN - 1347-6947

pISSN - 0916-8451

DOI - 10.1271/bbb.64.2731

Subject(s) - protein subunit , mutagenesis , chemistry , site directed mutagenesis , trypsin , chymotrypsin , sequence (biology) , biochemistry , peptide , enzyme , mutation , gene , mutant

Enterostatin (VPDPR), having anoretic and hypocholesterolemic activities, and its homologue LPYPR, a hypocholesterolemic peptide found in the glycinin A5A4B3 subunit, were introduced into the corresponding site (TNGPQ) of the proglycinin A1aB1b subunit by site-directed mutagenesis. Modified proglycinins were expressed in E. coli and recovered from the insoluble fraction. VPDPR and LPYPR were released by the action of chymotrypsin and trypsin as expected. The overall yields of purified VPDPR and LPYPR were 40% and 62%, respectively.

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