cDNA Cloning of the Cry1Aa Receptor Variants fromBombyx moriand Their Expression in Mammalian Cells | Zendy

Satoshi Ikawa | Zendy; Yoko TSUDA | Zendy; Takashi Fukada | Zendy; Kenji Sugimoto | Zendy; Michio Himeno | Zendy

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cDNA Cloning of the Cry1Aa Receptor Variants fromBombyx moriand Their Expression in Mammalian Cells

Author(s) -

Satoshi Ikawa,

Yoko TSUDA,

Takashi Fukada,

Kenji Sugimoto,

Michio Himeno

Publication year - 2000

Publication title -

bioscience biotechnology and biochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.509

H-Index - 116

eISSN - 1347-6947

pISSN - 0916-8451

DOI - 10.1271/bbb.64.2682

Subject(s) - bombyx mori , complementary dna , cloning (programming) , biology , microbiology and biotechnology , bombyx , gene , molecular cloning , genetics , computer science , programming language

We cloned cDNA of three variants of BtR175, a putative Bombyx mori receptor for Bacillus thuringiensis Cry1Aa delta-endotoxin by PCR. These variants were likely to be allelic to BtR175. cDNA of BtR175b, the most distant variant from BtR175, was introduced into mammalian cells. BtR175b protein was expressed in the plasma membrane of the cells and showed binding activity to Cry1Aa.

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