Staurosporine Promotion of Formation of Continuous Monolayers of Primary Rat Hepatocytes by Improving Attachment and Spreading | Zendy

Sumio Maeda | Zendy; Kong-Hua LIN | Zendy; Hidetoshi Inagaki | Zendy; Takao Saito | Zendy

Open Access

Staurosporine Promotion of Formation of Continuous Monolayers of Primary Rat Hepatocytes by Improving Attachment and Spreading

Author(s) -

Sumio Maeda,

Kong-Hua LIN,

Hidetoshi Inagaki,

Takao Saito

Publication year - 2000

Publication title -

bioscience biotechnology and biochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.509

H-Index - 116

eISSN - 1347-6947

pISSN - 0916-8451

DOI - 10.1271/bbb.64.1985

Subject(s) - monolayer , staurosporine , polarity (international relations) , hepatocyte , microbiology and biotechnology , biophysics , cell , chemistry , biology , biochemistry , enzyme , in vitro , protein kinase a

Primary rat hepatocytes form discontinuous monolayers even at their maximum density. Here, we show that staurosporine promotes attachment and spreading of hepatocytes onto culture substrates, so that hepatocytes form a close, continuous monolayer. This treatment did not attenuate major hepatic functions. Therefore, this technique is promising for making seamless cell-sheet structures, which will be applicable for cell-polarity experiments or artificial liver construction.

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