Substrate Specificity of Regiospecific Desaturation of Aliphatic Compounds by a MutantRhodococcusStrain | Zendy

K Koike | Zendy; Mikio Takaiwa | Zendy; Yoshiharu Kimura | Zendy; Shigeo Inoue | Zendy; Susumu Ito | Zendy

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Substrate Specificity of Regiospecific Desaturation of Aliphatic Compounds by a MutantRhodococcusStrain

Author(s) -

K Koike,

Mikio Takaiwa,

Yoshiharu Kimura,

Shigeo Inoue,

Susumu Ito

Publication year - 2000

Publication title -

bioscience biotechnology and biochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.509

H-Index - 116

eISSN - 1347-6947

pISSN - 0916-8451

DOI - 10.1271/bbb.64.1064

Subject(s) - rhodococcus , double bond , strain (injury) , chemistry , palmitic acid , substrate (aquarium) , fatty acid , mutant , alkyl , stereochemistry , substrate specificity , organic chemistry , carbon chain , biochemistry , enzyme , biology , gene , ecology , anatomy

Substrate specificity of cis-desaturation of alipahtic compounds by resting cells of a mutant, Rhodococcus sp. strain KSM-MT66, was examined. Among substrates tested, the rhodococcal cells were able to convert n-alkanes (C13-C19), 1-chloroalkanes (C16 and C18), ethyl fatty acids (C14-C17) and alkyl (C1-C4) esters of palmitic acid to their corresponding unsaturated products of cis configuration. The products from n-alkanes and 1-chloroalkanes had a double bond mainly at the 9th carbon from their terminal methyl groups, and the products from acyl fatty acids had a double bond mainly at the 6th carbon from their carbonyl carbons.

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