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The pH-stat fed-batch culture of Pseudomonas aeruginosa YPJ-80 was done to produce a rhamnolipid biosurfactant. With glucose as the sole carbon source, the final concentrations of cells and rhamnolipid biosurfactant obtained in 25 h were 25 g cell dry weight/l and 4.4 g/l, respectively.

Production of Rhamnolipid Biosurfactant by Fed-batch Culture ofPseudomonas aeruginosaUsing Glucose as a Sole Carbon Source