Production of Biodegradable Polyester by a Transgenic Tobacco | Zendy

Hideo Nakashita | Zendy; Yūkō Arai | Zendy; Keiko Yoshioka | Zendy; Toshiaki Fukui | Zendy; Yoshiharu Doi | Zendy; Ron Usami | Zendy; Kohki HORIKOSHI | Zendy; Isamu Yamaguchi | Zendy

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Production of Biodegradable Polyester by a Transgenic Tobacco

Author(s) -

Hideo Nakashita,

Yūkō Arai,

Keiko Yoshioka,

Toshiaki Fukui,

Yoshiharu Doi,

Ron Usami,

Kohki HORIKOSHI,

Isamu Yamaguchi

Publication year - 1999

Publication title -

bioscience biotechnology and biochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.509

H-Index - 116

eISSN - 1347-6947

pISSN - 0916-8451

DOI - 10.1271/bbb.63.870

Subject(s) - transgene , ralstonia , transformation (genetics) , aeromonas caviae , genetically modified crops , reductase , chemistry , gene , enzyme , biology , biochemistry , vibrionaceae

The acetoacetyl-CoA reductase gene (phbB) of Ralstonia eutropha and the poly[(R)-(-)-3-hydroxyalkanoate] synthase gene (phaCAC) of Aeromonas caviae were introduced into tobacco plant by Agrobacterium mediated transformation method. The resulting transgenic tobacco expressed both introduced genes and the expression of these genes was confirmed by enzymatic analysis and western blotting. GC-MS analysis of the chloroform extract of tobacco leaves indicated that the transgenic plant produced biodegradable polyester, poly-[(R)-(-)-3-hydroxybutyrate]. GPC analysis indicated that the number-average molecular weights (Mn) and polydispersity (Mw/Mn) were 32,000 and 1.90, respectively.

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