Construction of an Effective Host-Vector System for the YeastSaccharomyces exiguusYp74L-3 | Zendy

Taisuke Hisatomi | Zendy; K. Takahashi | Zendy; Tetsuya OOMOTO | Zendy; Yasunori Yamashita | Zendy; Shuichi Hasegawa | Zendy; Michio Tsuboi | Zendy

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Construction of an Effective Host-Vector System for the YeastSaccharomyces exiguusYp74L-3

Author(s) -

Taisuke Hisatomi,

K. Takahashi,

Tetsuya OOMOTO,

Yasunori Yamashita,

Shuichi Hasegawa,

Michio Tsuboi

Publication year - 1999

Publication title -

bioscience biotechnology and biochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.509

H-Index - 116

eISSN - 1347-6947

pISSN - 0916-8451

DOI - 10.1271/bbb.63.847

Subject(s) - ura3 , vector (molecular biology) , biology , plasmid , host (biology) , saccharomyces cerevisiae , genetics , strain (injury) , yeast , gene , recombinant dna , anatomy

An effective host-vector system specific to the yeast Saccharomyces exiguus Yp74L-3 was constructed to promote the molecular genetic analyses for the yeast. To obtain a stable reversionless host strain, we constructed an S. exiguus strain carrying leu2::ScURA3 by disrupting the S. exiguus LEU2 gene with the S. cerevisiae URA3 gene. A vector plasmid unique to S. exiguus was subsequently developed by inserting both the LEU2 gene and an ARS cloned from S. exiguus into an Escherichia coli phagemid, pUC119. The vector constructed, pTH119 was able to transform the S. exiguus leu2::ScURA3 strain to Leu+ efficiently. The stability of the vector in the S. exiguus host cells resembled that of a YRp-type vector in S. cerevisiae.

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