Molecular Cloning and Characterization of a cDNA for an Iron-Superoxide Dismutase in Rice (Oryza sativaL.) | Zendy

Hironori Kaminaka | Zendy; Shigeto Morita | Zendy; Megumi Tokumoto | Zendy; Hidefumi Yokoyama | Zendy; Takehiro Masumura | Zendy; Kunisuke Tanaka | Zendy

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Molecular Cloning and Characterization of a cDNA for an Iron-Superoxide Dismutase in Rice (Oryza sativaL.)

Author(s) -

Hironori Kaminaka,

Shigeto Morita,

Megumi Tokumoto,

Hidefumi Yokoyama,

Takehiro Masumura,

Kunisuke Tanaka

Publication year - 1999

Publication title -

bioscience biotechnology and biochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.509

H-Index - 116

eISSN - 1347-6947

pISSN - 0916-8451

DOI - 10.1271/bbb.63.302

Subject(s) - complementary dna , oryza sativa , peptide sequence , amino acid , biochemistry , superoxide dismutase , biology , cdna library , microbiology and biotechnology , cloning (programming) , peptide , molecular cloning , gene , enzyme , computer science , programming language

We have isolated a cDNA encoding Fe-SOD from rice (Oryza sativa L.). The deduced amino acid sequence consists of a polypeptide with 255 amino acids, including a putative transit peptide (40 a.a.) in amino-terminal residues. This sequence is similar to the known plant Fe-SODs but not classified in the group of known Fe-SODs. The metal analysis and SOD assays of the partial purified recombinant protein expressed in E. coli showed that this cDNA encodes an iron-containing SOD. However this SOD activity was not inhibited by the treatment with hydrogen peroxide, which was expected to inhibit known Fe-SOD activity. mRNA of rice Fe-SOD was detected in all vegetative tissues examined, being especially abundant in calli, and strongly increased by light induction. These results suggested that this cDNA encodes rice Fe-SOD, which is apparently distinct from known plant Fe-SODs.

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