Open AccessSubsite Structure of α-Amylase II fromThermoactinomyces vulgarisR-47
Author(s) -
Yoichiro Shimura,
Qiong Wang,
Yoshiyuki Sakano
Publication year - 1999
Publication title -
bioscience biotechnology and biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.509
H-Index - 116
eISSN - 1347-6947
pISSN - 0916-8451
DOI - 10.1271/bbb.63.2199
Subject(s) - affinities , chemistry , amylase , stereochemistry , catalysis , biochemistry , enzyme
We estimated the subsite structure of α-amylase II (TVA II) from Thermoactinomyces vulgaris R-47 expressed in Escherichia coli. TVA II has eight subsites, and the catalytic site is between the 5th and 6th subsite from the non-reducing end side. The subsite affinities, A-5, A-4, A-3, A-2, (A-1+A+1), A+2, and A+3, were calculated to be -0.35, 0.93, 0.55, 2.56, 1.18, 1.71, and 0.01 kcal mol(-1), respectively.
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