Inhibition of Plant Transformation by Phytolaccoside B fromPhytolacca americanaCallus | Zendy

Hiroshi Kanzaki | Zendy; Toshihiko KAGEMORI | Zendy; Yoko YAMACHIKA | Zendy; Teruhiko Nitoda | Zendy; Kazuyoshi Kawazu | Zendy

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Inhibition of Plant Transformation by Phytolaccoside B fromPhytolacca americanaCallus

Author(s) -

Hiroshi Kanzaki,

Toshihiko KAGEMORI,

Yoko YAMACHIKA,

Teruhiko Nitoda,

Kazuyoshi Kawazu

Publication year - 1999

Publication title -

bioscience biotechnology and biochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.509

H-Index - 116

eISSN - 1347-6947

pISSN - 0916-8451

DOI - 10.1271/bbb.63.1657

Subject(s) - callus , transformation (genetics) , triterpene , bioassay , glycoside , botany , biology , traditional medicine , chemistry , biochemistry , gene , genetics , pathology , medicine , alternative medicine

The newly established GUS expression bioassay on the callus extracts of 22 species of plants revealed that the methanol extract of Phytolacca americana callus had the most potent inhibitory activity against agrobacterial plant transformation. A triterpene glycoside phytolaccoside B was isolated from the extract as a genuine plant transformation inhibitor having neither antiagrobacterial nor phytotoxic activity. This compound is promising for use as a biochemical probe for studies on the plant transformation mechanism.

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