Determination of a Small Quantity of Cystine in the Presence of a Large Amount of Cysteine | Zendy

Shuzo Yamagata | Zendy; Tomonori Iwama | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Open Access

Determination of a Small Quantity of Cystine in the Presence of a Large Amount of Cysteine

Author(s) -

Shuzo Yamagata,

Tomonori Iwama

Publication year - 1999

Publication title -

bioscience biotechnology and biochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.509

H-Index - 116

eISSN - 1347-6947

pISSN - 0916-8451

DOI - 10.1271/bbb.63.1503

Subject(s) - cystine , cysteine , ninhydrin , dithiothreitol , chemistry , reagent , cystinuria , homocysteine , biochemistry , chromatography , organic chemistry , amino acid , enzyme

A procedure is described to precisely determine a very small amount of cystine in the presence of a large amount of cysteine. After completely modifying cysteine with N-ethylmaleimide, the remaining reagent was reacted with DL-homocysteine. Cystine was determined, after being reduced with dithiothreitol, by the reaction with ninhydrin carried out under acidic conditions. The procedure makes it possible to precisely determine the amount of cystine present with cysteine in a concentration ratio of 1:2,000. By employing this procedure, auto-oxidation of cysteine to cystine in a mixture for the L-cysteine α,β-elimination reaction was investigated.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research