Preparation of 4-(4′-Hydroxyanilino)-5-anilinophthalimide and 4,5-Bis-(4′-hydroxyanilino)-phthalimide by Microbial Hydroxylation | Zendy

Stefan Weidner | Zendy; K. Goeke | Zendy; Uwe Trinks | Zendy; Peter Traxler | Zendy; Katharina Ucci-Stoll | Zendy; Oreste Ghisalba | Zendy

Open Access

Preparation of 4-(4′-Hydroxyanilino)-5-anilinophthalimide and 4,5-Bis-(4′-hydroxyanilino)-phthalimide by Microbial Hydroxylation

Author(s) -

Stefan Weidner,

K. Goeke,

Uwe Trinks,

Peter Traxler,

Katharina Ucci-Stoll,

Oreste Ghisalba

Publication year - 1999

Publication title -

bioscience biotechnology and biochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.509

H-Index - 116

eISSN - 1347-6947

pISSN - 0916-8451

DOI - 10.1271/bbb.63.1497

Subject(s) - biotransformation , hydroxylation , beauveria bassiana , phthalimide , chitinase , chemistry , streptomyces , biocatalysis , strain (injury) , stereochemistry , derivative (finance) , enzyme , organic chemistry , biochemistry , microbiology and biotechnology , biology , bacteria , botany , reaction mechanism , biological pest control , genetics , anatomy , catalysis , financial economics , economics

A microbial screening indicated that two fungal strains, Beauveria bassiana DSM 1344=ATCC 7159 and Cunninghamella elegans DSM 1908=ATCC 9245, as well as four bacterial strains belonging to the genus Streptomyces were able to hydroxylate 4,5-dianilinophthalimide (DAPH, CGP52411) to 4-(4'-hydroxyanilino)-5-anilinophthalimide. Cunninghamella elegans DSM 1908 turned out to be the most active biocatalyst and was also able to form the dihydroxy derivative, 4,5-bis(4'-hydroxyanilino)phthalimide. The reaction for the monohydroxylated biotransformation product was carried out on a preparative scale, and the culture conditions for the formation of 4-(4'-hydroxy- anilino)-5-anilinophthalimide with this strain were op-timized.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research