Cloning and Sequencing of the β-Fructofuranosidase gene fromBacillussp. V230 | Zendy

Masashi Kurimoto | Zendy; Keiji Tsusaki | Zendy; Michio Kubota | Zendy; Shigeharu Fukuda | Zendy; Yoshio Tsujisaka | Zendy

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Cloning and Sequencing of the β-Fructofuranosidase gene fromBacillussp. V230

Author(s) -

Masashi Kurimoto,

Keiji Tsusaki,

Michio Kubota,

Shigeharu Fukuda,

Yoshio Tsujisaka

Publication year - 1999

Publication title -

bioscience biotechnology and biochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.509

H-Index - 116

eISSN - 1347-6947

pISSN - 0916-8451

DOI - 10.1271/bbb.63.1107

Subject(s) - zymomonas mobilis , bacillus subtilis , nucleic acid sequence , biology , gene , levansucrase , escherichia coli , cloning (programming) , peptide sequence , gene product , biochemistry , bacteria , genetics , fermentation , gene expression , programming language , ethanol fuel , computer science

The beta-fructofuranosidase gene (bff) from Bacillus sp. V230 has been cloned in Escherichia coli and its nucleotide sequence has been analyzed. The product of bff consists of a signal sequence of 32 amino acid (a.a.) residues for secretion and 455 a.a. residues of the extracellular beta-fructofuranosidase. The a.a. sequence of the bff product has similarities with those of the Bacillus subtilis levanscrase (63.7% identity), the Streptococcus mutans fructosyltransferase (33.7%), and the Zymomonas mobilis levanscrase and beta-fructofuranosidase (15%).

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