Purification and Characterization of RecombinantMortierella vinaceaα-Galactosidases I and II Expressed inSaccharomyces cerevisiae | Zendy

Hajime Shibuya | Zendy; Hideyuki Kobayashi | Zendy; Shigeki Yoshida | Zendy; Satoshi Kaneko | Zendy; Gwi Gun Park | Zendy; Isao Kusakabe | Zendy

Open Access

Purification and Characterization of RecombinantMortierella vinaceaα-Galactosidases I and II Expressed inSaccharomyces cerevisiae

Author(s) -

Hajime Shibuya,

Hideyuki Kobayashi,

Shigeki Yoshida,

Satoshi Kaneko,

Gwi Gun Park,

Isao Kusakabe

Publication year - 1999

Publication title -

bioscience biotechnology and biochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.509

H-Index - 116

eISSN - 1347-6947

pISSN - 0916-8451

DOI - 10.1271/bbb.63.1096

Subject(s) - saccharomyces cerevisiae , recombinant dna , yeast , enzyme , galactosidases , biochemistry , biology , chemistry , gene , beta galactosidase , escherichia coli

The cDNAs coding for Mortierella vinacea alpha-galactosidases I and II were expressed in Saccharomyces cerevisiae under the control of the yeast GAL10 promoter. The recombinant enzymes purified to homogeneity from the culture filtrate were glycosylated, and had properties identical to those of the native enzymes except for improving the heat stability of alpha-galactosidase II and decreasing the specific activities of both enzymes.

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