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Purification ofPomacea canaliculataα-Fucosidase Isoforms with Different Thermostabilities
Author(s) -
Koji Hirata,
Yoichi Aso,
Seiji Yasuda,
Masatsune Ishiguro
Publication year - 1998
Publication title -
bioscience biotechnology and biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.509
H-Index - 116
eISSN - 1347-6947
pISSN - 0916-8451
DOI - 10.1271/bbb.62.807
Subject(s) - pomacea canaliculata , thermostability , gene isoform , enzyme , biochemistry , biology , fucosidase , chemistry , glycoprotein , gene , ecology , snail , fucose
Two isoforms, F1 (pI 4.7) and F2 (pI 4.9), of α-fucosidase were purified from the viscera of Pomacea canaliculata. The thermostability of F1 was higher than that of F2. Both were 260-kDa proteins containing polypeptides (55 and 52 kDa) and sugar. There were small differences between F1 and F2 in the optimum conditions for the enzyme reaction and pH stability.

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