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A transgalactosylation reaction from p-nitrophenyl-α-D-galactopyranoside to 1-deoxynojirimycin was done using α-galactosidase [EC 3.2.1.22] from green coffee beans. The enzyme formed 6-O-α-D-galactopyranosyl-1-deoxynojirimycin as the major product.

Enzymatic Synthesis of 6-O-.ALPHA.-D-Galactopyranosyl-1-deoxynojirimycin Using .ALPHA.-Galactosidase from Green Coffee Beans.