Enzymatic Synthesis of 6-O-.ALPHA.-D-Galactopyranosyl-1-deoxynojirimycin Using .ALPHA.-Galactosidase from Green Coffee Beans.
Author(s) -
Nam Soo Paek,
Dae Jung Kang,
HongSub Lee,
Jeong Yong Lee,
Yong Jin Choi,
Tae Han Kim,
Kee Won Kim
Publication year - 1998
Publication title -
bioscience biotechnology and biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.509
H-Index - 116
eISSN - 1347-6947
pISSN - 0916-8451
DOI - 10.1271/bbb.62.588
Subject(s) - alpha (finance) , alpha galactosidase , alpha glucosidase , enzyme , chemistry , biochemistry , beta galactosidase , food science , mathematics , medicine , gene expression , gene , fabry disease , construct validity , statistics , disease , pathology , psychometrics
A transgalactosylation reaction from p-nitrophenyl-α-D-galactopyranoside to 1-deoxynojirimycin was done using α-galactosidase [EC 3.2.1.22] from green coffee beans. The enzyme formed 6-O-α-D-galactopyranosyl-1-deoxynojirimycin as the major product.
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