cDNA Cloning, Expression, and Mutagenesis of Scytalone Dehydratase Needed for Pathogenicity of the Rice Blast Fungus, Pyricularia oryzae. | Zendy

Takayuki Motoyama | Zendy; Kinya IMANISHI | Zendy; Isamu Yamaguchi | Zendy

Open Access

cDNA Cloning, Expression, and Mutagenesis of Scytalone Dehydratase Needed for Pathogenicity of the Rice Blast Fungus, Pyricularia oryzae.

Author(s) -

Takayuki Motoyama,

Kinya IMANISHI,

Isamu Yamaguchi

Publication year - 1998

Publication title -

bioscience biotechnology and biochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.509

H-Index - 116

eISSN - 1347-6947

pISSN - 0916-8451

DOI - 10.1271/bbb.62.564

Subject(s) - pyricularia , biology , complementary dna , dehydratase , fungus , peptide sequence , gene , cloning (programming) , heterologous expression , microbiology and biotechnology , genetics , recombinant dna , botany , computer science , programming language

We purified scytalone dehydratase from the rice blast fungus, Pyricularia oryzae, and cloned its cDNA on the basis of its amino acid sequence. The deduced amino acid sequence was 62% identical to the scytalone dehydratase from Colletotrichum lagenarium. The expression of this gene was induced transcriptionally in the stationary phase when melanin synthesis occurs. We constructed a heterologous expression system for the enzyme in Escherichia coli, did deletion analysis with this system, and found that a C-terminal region is essential for the enzyme function.

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