Cloning Genomic DNA Encoding Apple Polyphenol Oxidase and Comparison of the Gene Product inEscherichia coliand in Apple | Zendy

Miyoshi Haruta | Zendy; Masatsune Murata | Zendy; Ayami Hiraide | Zendy; Hiroshi Kadokura | Zendy; Makari Yamasaki | Zendy; Masaaki Sakuta | Zendy; Seki Shimizu | Zendy; Seiichi Homma | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Open Access

Cloning Genomic DNA Encoding Apple Polyphenol Oxidase and Comparison of the Gene Product inEscherichia coliand in Apple

Author(s) -

Miyoshi Haruta,

Masatsune Murata,

Ayami Hiraide,

Hiroshi Kadokura,

Makari Yamasaki,

Masaaki Sakuta,

Seki Shimizu,

Seiichi Homma

Publication year - 1998

Publication title -

bioscience biotechnology and biochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.509

H-Index - 116

eISSN - 1347-6947

pISSN - 0916-8451

DOI - 10.1271/bbb.62.358

Subject(s) - genomic dna , escherichia coli , polyphenol oxidase , gene , biology , dna , cloning (programming) , microbiology and biotechnology , biochemistry , enzyme , peroxidase , computer science , programming language

Two PCR-amplified genomic DNA fragments encoding apple (cv. Fuji) polyphenol oxidase (PPO) were cloned and sequenced. A comparison of genomic DNA with cDNAs revealed that the PPOs lacked introns. Both PPO DNAs appear to encode a 66-kDa precursor protein consisting of a 56-kDa mature protein and a N-terminal transit peptide of 10-kDa N-terminal transit peptide. Apple PPO DNA was expressed in Escherichia coli, and the gene product (56 kDa) without a transit peptide was immunochemically detected and was the same size (ca. 65 kDa) as the main PPO of apple fruit by SDS-PAGE.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research