Identification of a Structural Gene Encoding a Metallothionein-like Domain that Includes a Putative Regulator Protein forStreptomycesProtease Gene Expression | Zendy

Seiichi Taguchi | Zendy; Takahiro Ogawa | Zendy; Takeshi Endo | Zendy; Haruo Momose | Zendy

Open Access

Identification of a Structural Gene Encoding a Metallothionein-like Domain that Includes a Putative Regulator Protein forStreptomycesProtease Gene Expression

Author(s) -

Seiichi Taguchi,

Takahiro Ogawa,

Takeshi Endo,

Haruo Momose

Publication year - 1998

Publication title -

bioscience biotechnology and biochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.509

H-Index - 116

eISSN - 1347-6947

pISSN - 0916-8451

DOI - 10.1271/bbb.62.2476

Subject(s) - open reading frame , gene , biology , frameshift mutation , regulator gene , microbiology and biotechnology , terminator (solar) , streptomyces , structural gene , genetics , gene product , reporter gene , regulation of gene expression , gene expression , mutation , peptide sequence , mutant , ionosphere , physics , astronomy , bacteria

An open reading frame (termed ORF-PR) encoding a metallothionein-like domain-including protein was found upstream of a previously identified Streptomyces chymotrypsin-type protease gene (sam-P20). Promoter and terminator activities of ORF-PR were detected using the promoterless Streptomyces tyrosinase gene as a reporter gene and expression of ORF-PR was supposed to occur before that of sam-P20 gene. Frameshift mutation analysis showed that the ORF-PR product might act as a repressive regulator of the sam-P20 gene.

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