Fluorescent Determination of Double-stranded DNA with an Anionic 1,1′-Azonaphthalene Derivative | Zendy

Yasumasa Fukushima | Zendy; Mayumi HAYASHI | Zendy; Hiroshi Nakajima | Zendy

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Fluorescent Determination of Double-stranded DNA with an Anionic 1,1′-Azonaphthalene Derivative

Author(s) -

Yasumasa Fukushima,

Mayumi HAYASHI,

Hiroshi Nakajima

Publication year - 1998

Publication title -

bioscience biotechnology and biochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.509

H-Index - 116

eISSN - 1347-6947

pISSN - 0916-8451

DOI - 10.1271/bbb.62.2254

Subject(s) - fluorescence , dna , homogeneous , aqueous solution , derivative (finance) , double stranded , chemistry , biophysics , base pair , biochemistry , biology , optics , physics , financial economics , economics , thermodynamics

Palatine chrome black 6BN (PCB6BN) is virtually non-fluorescent in an aqueous solution or in the presence of single-stranded DNA (ssDNA), whereas the fluorescence intensity of PCB6BN was linearly enhanced up to 300 μM of double-stranded DNA (dsDNA) base pairs. PCB6BN could be a useful fluorescent probe for quantifying dsDNA even when ssDNA is present for both heterogeneous and homogeneous assays.

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