Identification and Characterization ofAcinetobactersp. CNU961 Able to Grow with Phenol at High Concentrations | Zendy

Kwangcheol C. Jeong | Zendy; Eun Ye Jeong | Zendy; Tay Eak Hwang | Zendy; Sang Ho Choi | Zendy

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Identification and Characterization ofAcinetobactersp. CNU961 Able to Grow with Phenol at High Concentrations

Author(s) -

Kwangcheol C. Jeong,

Eun Ye Jeong,

Tay Eak Hwang,

Sang Ho Choi

Publication year - 1998

Publication title -

bioscience biotechnology and biochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.509

H-Index - 116

eISSN - 1347-6947

pISSN - 0916-8451

DOI - 10.1271/bbb.62.1830

Subject(s) - catechol , phenol , hydroxylation , complementation , enzyme , biochemistry , chemistry , strain (injury) , phenols , stereochemistry , biology , organic chemistry , gene , mutant , anatomy

An Acinetobacter sp., strain CNU961, with a higher tolerance to phenol was isolated, and identified through a set of taxonomic studies and a genetic complementation test. Enzymatic and mutagenic studies found that the strain dissimilate phenol by hydroxylation to catechol followed by an ortho-ring cleavage pathway to further mineralize it. The phenol hydroxylase, which is an inducible enzyme and requires NADPH for optimum activity, was not inhibited by phenol at concentrations up to 0.5 mM. The different kinetic behaviors of the enzyme activities on NADPH and on phenol reflected that the phenol hydroxylase of strain CNU961 is a multisubunit allosteric enzyme consisting of heterogeneous polypeptides.

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