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The application of the on-line HPLC-exciton CD analysis with (S)-TBMB-carboxylic acid that simultaneously determined the enantiomeric composition and absolute configuration of vicinal diols, diamines and amino alcohols to 1,3-diols was studied. All optical isomers of di-O-(S)-TBMB-carbonyl-1,3-butanediols and 2,4-pentanediols were separated and their absolute configurations were determined without reference samples on line by HPLC.

bioscience, biotechnology, and biochemistry

Study on the Application of the (<i>S</i>)-2-<i>tert</i>-Butyl-2-methyl-1,3-benzodioxole-4-carboxylic acid-based On-line HPLC-Exciton CD Analysis to Acyclic 1,3-Diols

Study on the Application of the (S)-2-tert-Butyl-2-methyl-1,3-benzodioxole-4-carboxylic acid-based On-line HPLC-Exciton CD Analysis to Acyclic 1,3-Diols