Structures of Novel Acidic Galactooligosaccharides Synthesized byBacillus circulansβ-Galactosidase | Zendy

Shūichi Yanahira | Zendy; Yukiko YABE | Zendy; Masamichi Nakakoshi | Zendy; Susumu Miura | Zendy; Noritaka MATSUBARA | Zendy; Hidetoshi Ishikawa | Zendy

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Structures of Novel Acidic Galactooligosaccharides Synthesized byBacillus circulansβ-Galactosidase

Author(s) -

Shūichi Yanahira,

Yukiko YABE,

Masamichi Nakakoshi,

Susumu Miura,

Noritaka MATSUBARA,

Hidetoshi Ishikawa

Publication year - 1998

Publication title -

bioscience biotechnology and biochemistry

Language(s) - Uncategorized

Resource type - Journals

SCImago Journal Rank - 0.509

H-Index - 116

eISSN - 1347-6947

pISSN - 0916-8451

DOI - 10.1271/bbb.62.1791

Subject(s) - bacillus circulans , chemistry , biochemistry , microbiology and biotechnology , biology , enzyme

The structures of acidic oligosaccharides synthesized by a transglycosylation reaction by Bacillus circulans beta-galactosidase, using lactose as the galactosyl donor, and N-acetylneuraminic acid (NeuAc) and glucuronic acid (GlcUA) as the acceptors were investigated. Acidic oligosaccharides thus synthesized were purified by anion exchange chromatography and charcoal chromatography. The MS and NMR studies indicated that the acidic oligosaccharides from NeuAc were Gal beta-(1-->8)-NeuAc, Gal beta-(1-->9)-NeuAc, and Gal beta-(1-->3)-Gal beta-(1-->8)-NeuAc, and those from GlcUA were Gal beta-(1-->3)-GlcUA and Gal beta-(1-->4)-Gal beta-(1-->3)-GlcUA. These are novel acidic galactooligosaccharides.

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