Expression ofAspergillus aculeatusNo. F-50 Cellobiohydrolase I (cbhI) and β-glucosidase 1 (bgl1) genes bySaccharomyces cerevisiae | Zendy

Goro Takada | Zendy; Takashi Kawaguchi | Zendy; Junichi Sumitani | Zendy; Motoo Arai | Zendy

Open Access

Expression ofAspergillus aculeatusNo. F-50 Cellobiohydrolase I (cbhI) and β-glucosidase 1 (bgl1) genes bySaccharomyces cerevisiae

Author(s) -

Goro Takada,

Takashi Kawaguchi,

Junichi Sumitani,

Motoo Arai

Publication year - 1998

Publication title -

bioscience biotechnology and biochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.509

H-Index - 116

eISSN - 1347-6947

pISSN - 0916-8451

DOI - 10.1271/bbb.62.1615

Subject(s) - recombinant dna , cellulase , saccharomyces cerevisiae , gene , enzyme , biology , chemistry , microbiology and biotechnology , biochemistry

A cellobiohydrolase I (cbhI) and a beta-glucosidase 1 (bgl1) gene of Aspergillus aculeatus were expressed in Saccharomyces cerevisiae. The transformed cells secreted the enzymes efficiently in an active form. The recombinant CBHI gave two bands of different molecular mass (110 and 90 kDa) and the recombinant BGL1 gave one band (180 kDa) by SDS-PAGE. The recombinant CBHI and BGL1 had the same enzymatical properties as the native enzyme except for the specific activity toward cellulosic substrates. By the combination of three different types of cellulases, FI-CMCase, CBHI, and BGL1, we could hydrolyze Avicel up to 59% under our experimental conditions.

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