Occurrence of Free N-Glycans in Pea (Pisum sativum, L) Seedlings† | Zendy

Yoshinobu Kimura | Zendy; Shigeaki Takagi | Zendy; Tomonori Shiraishi | Zendy

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Occurrence of Free N-Glycans in Pea (Pisum sativum, L) Seedlings^†

Author(s) -

Yoshinobu Kimura,

Shigeaki Takagi,

Tomonori Shiraishi

Publication year - 1997

Publication title -

bioscience biotechnology and biochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.509

H-Index - 116

eISSN - 1347-6947

pISSN - 0916-8451

DOI - 10.1271/bbb.61.924

Subject(s) - pisum , sativum , glycan , size exclusion chromatography , fractionation , chemistry , glycoprotein , chromatography , residue (chemistry) , biochemistry , biology , botany , enzyme

Free N-glycans have been found in pea seedlings. These free N-glycans were coupled with 2-aminopyridine and purified by gel filtration, Con Α-Sepharose affinity chromatography, and size fractionation HPLC. These structures of pyridylaminated free N-glycans were analyzed by exomannosidase digestions and ion- spray tandem mass spectrometry. The structural analyses showed that the several oligomannose-type sugar chains having one GlcNAc residue at the reducing-end side occur in the seedlings, suggesting the endo-β-N-acetylgIucosaminidase PS [Y. Kimura et al., Biosci. Biotech. Biochem., 60, 228-232 (1996)] should be involved in the release of oligomannose-type N-glycans from the storage glycoproteins [Y. Kimura et al., Biosci. Biotech. Biochem., 60, 1841-1850 (1996)] during the germination of pea seeds.

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