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To investigate the transformation from (2E,6E)-farnesyl pyrophosphate to (2Z,4E)-γ-ionylideneethanol in the abscisic acid-producing fungi, Cercospora cruenta, plausible [2- 14 C]-C 15 intermediates were prepared and fed. Substrates such as (2E,6E)-farnesyl pyrophosphate, (2Z,4E)-γ-ionylideneethanol and its pyrophosphate were incorporated into ABA and its known biosynthetic precursors. It is suggested that (2E,6E&gt;farnesyl pyrophosphate is converted to (2Z,4E)-γ-ionylideneethanol in four consecutive steps: dehydrogenation, isomerization, cyclization and hydrolysis.

Incorporation of Farnesyl Pyrophosphate Derivertives into Abscisic Acid and Its Biosynthetic Intermediates in Cevcospova cruenta