Growth Inhibition, Morphological Change, and Ectoenzyme Release of LLC-PK1 Cells by Phosphatidylinositol-specific Phospholipase C ofBacillus thuringiensis | Zendy

Chisako Itami | Zendy; Yukio Kimura | Zendy; Ryo Taguchi | Zendy; Hiroh Ikezawa | Zendy; Toshikatsu Nakabayashi | Zendy

Open Access

Growth Inhibition, Morphological Change, and Ectoenzyme Release of LLC-PK1 Cells by Phosphatidylinositol-specific Phospholipase C ofBacillus thuringiensis

Author(s) -

Chisako Itami,

Yukio Kimura,

Ryo Taguchi,

Hiroh Ikezawa,

Toshikatsu Nakabayashi

Publication year - 1997

Publication title -

bioscience biotechnology and biochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.509

H-Index - 116

eISSN - 1347-6947

pISSN - 0916-8451

DOI - 10.1271/bbb.61.776

Subject(s) - phospholipase c , phosphatidylinositol , bacillus thuringiensis , alkaline phosphatase , pi , biochemistry , biology , cell growth , cell , cell culture , phosphodiesterase , microbiology and biotechnology , enzyme , bacteria , signal transduction , genetics

Phosphatidylinositol-specific phospholipase C (PI-PLC) from Bacillus thuringiensis added to a culture of LLC-PK1 cells inhibited cell growth by 40%. In contrast with normal cells, the cells cultured in the presence of PI-PLC showed needle-like appendages which seemed to have been formed due to portions of the cell remaining adhered to the culture dish as the cell shrank. When LLC-PK1 cells were treated with PI-PLC, significant amounts of alkaline phosphatase and alkaline phosphodiesterase I were released specifically from the apical surface of the LLC-PK1 cells. Furthermore, PI-PLC treatment caused a delay of enzyme production and dome formation. These data indicate that glycosyl-phosphatidylinositol (GPI)-anchored proteins on the surface of LLC-PK1 cells are important in cell growth and differentiation. Also, the combined use of LLC-PK1 cells and PI-PLC of B. thuringiensis is effective for investigating the function of GPI-anchor proteins.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research