Clarification of the Promoter Structure of the Osmoregulatedgpd1+Gene Encoding an Isozyme of NADH-dependent Glycerol-3-phosphate Dehydrogenase in Fission Yeast | Zendy

Ryusuke Ohmiya | Zendy; Hirofumi Aiba | Zendy; Hisami Yamada | Zendy; Takeshi Mizuno | Zendy

Open Access

Clarification of the Promoter Structure of the Osmoregulatedgpd1⁺Gene Encoding an Isozyme of NADH-dependent Glycerol-3-phosphate Dehydrogenase in Fission Yeast

Author(s) -

Ryusuke Ohmiya,

Hirofumi Aiba,

Hisami Yamada,

Takeshi Mizuno

Publication year - 1997

Publication title -

bioscience biotechnology and biochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.509

H-Index - 116

eISSN - 1347-6947

pISSN - 0916-8451

DOI - 10.1271/bbb.61.553

Subject(s) - schizosaccharomyces pombe , glycerol kinase , glycerol 3 phosphate dehydrogenase , dehydrogenase , biology , gene , transcription (linguistics) , isozyme , biochemistry , yeast , microbiology and biotechnology , enzyme , saccharomyces cerevisiae , linguistics , philosophy

The gpd1+ gene of Schizosaccharomyces pombe, encoding an isozyme of NADH-dependent glycerol-3-phosphate dehydrogenase, is responsible for an osmoinducible glycerol production in response to external high osmotic stimuli, and its expression is osmoregulated at the level of transcription. In this study, the structure of the osmoinducible promoter of gpd1+ is described. Not only the core promoter elements including the putative TATA-box and transcription start site, but also a short upstream activation sequence (UAS) was identified, which are involved in the osmotic induction of the gpd1+ gene through the Wis1 MAP (mitogen activated protein) kinase pathway.

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