High Efficiency Transformation ofBacillus brevisby Electroporation | Zendy

Akiko Okamoto | Zendy; Akihiko Kosugi | Zendy; Yukimichi KOIZUMI | Zendy; Fujiharu Yanagida | Zendy; Shigezo Udaka | Zendy

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High Efficiency Transformation ofBacillus brevisby Electroporation

Author(s) -

Akiko Okamoto,

Akihiko Kosugi,

Yukimichi KOIZUMI,

Fujiharu Yanagida,

Shigezo Udaka

Publication year - 1997

Publication title -

bioscience biotechnology and biochemistry

Language(s) - Uncategorized

Resource type - Journals

SCImago Journal Rank - 0.509

H-Index - 116

eISSN - 1347-6947

pISSN - 0916-8451

DOI - 10.1271/bbb.61.202

Subject(s) - electroporation , transformation (genetics) , plasmid , transformation efficiency , polyethylene glycol , dna , bacillus (shape) , microbiology and biotechnology , bacillaceae , chemistry , biology , bacteria , genetics , biochemistry , gene , bacillus subtilis , agrobacterium

Various conditions in the Bacillus brevis transformation by electroporation were investigated to increase the efficiency. Competent cell volume and presence of polyethylene glycol 6000 and single stranded DNA on pulsing were critical for the efficiency. As a result, we established the improved method by which 10(7)-10(8) transformants/microgram plasmid DNA could be obtained.

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