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The homoserine dehydrogenase (HD) genes from Brevibacterium lactofermentum lysine- and threonine-producing mutants were cloned, using the polymerase chain reaction, and sequenced. We found the amino acid substitutions, Val104Ile in the lysine-producing mutants in which HD may cause leaky mutation and Ser393Phe in the threonine-producing mutant with feedback-insensitive HD.

bioscience, biotechnology, and biochemistry

Sequence Analysis of Functional Regions of Homoserine Dehydrogenase Genes from<scp>l</scp>-Lysine and<scp>l</scp>-Threonine-producing Mutants of<i>Brevibacterium lactofermentum</i>

Sequence Analysis of Functional Regions of Homoserine Dehydrogenase Genes froml-Lysine andl-Threonine-producing Mutants ofBrevibacterium lactofermentum