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The effects of various plant growth regulators and nutrients on cell growth and anthraquinone production in cell suspension cultures of Rubia akane Nakai were investigated. Use of an optimized medium resulted in a two-fold increase in the anthraquinone content. From the cell cultures 1,2-dihydroxyanthraquinone was isolated as the main constituent.

Anthraquinone Production by Cell Suspension Cultures ofRubia akaneNakai