Transient Expression of Goat Growth Hormone Gene in Poplar (Populus albaL.) Protoplasts: A Quick Method for Detection of Foreign Gene Expression in mRNA Level | Zendy

Jingbo Qiao | Zendy; Yasuyuki Ishihara | Zendy; Hiroyuki Kuroda | Zendy; Fukumi Sakai | Zendy; Hiroshi Sakai | Zendy; Tohru Komano | Zendy

Open Access

Transient Expression of Goat Growth Hormone Gene in Poplar (Populus albaL.) Protoplasts: A Quick Method for Detection of Foreign Gene Expression in mRNA Level

Author(s) -

Jingbo Qiao,

Yasuyuki Ishihara,

Hiroyuki Kuroda,

Fukumi Sakai,

Hiroshi Sakai,

Tohru Komano

Publication year - 1997

Publication title -

bioscience biotechnology and biochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.509

H-Index - 116

eISSN - 1347-6947

pISSN - 0916-8451

DOI - 10.1271/bbb.61.1580

Subject(s) - electroporation , complementary dna , gene expression , gene , microbiology and biotechnology , reverse transcriptase , biology , messenger rna , polymerase chain reaction , protoplast , real time polymerase chain reaction , expression vector , reverse transcription polymerase chain reaction , transfection , dna , recombinant dna , genetics

We developed a sensitive, accurate, and fast method to detect foreign gene expression using reverse transcriptase-mediated polymerase chain reaction (RT-PCR) in poplar tree (Populus alba L.) protoplasts. Template total RNA was purified by removing the transfected foreign gene vector completely with DNase I treatment before the RT reaction. Expression of cDNA that encodes goat growth hormone was confirmed at the mRNA level 24 h after electroporation mediated DNA transfer.

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