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We developed a sensitive, accurate, and fast method to detect foreign gene expression using reverse transcriptase-mediated polymerase chain reaction (RT-PCR) in poplar tree (Populus alba L.) protoplasts. Template total RNA was purified by removing the transfected foreign gene vector completely with DNase I treatment before the RT reaction. Expression of cDNA that encodes goat growth hormone was confirmed at the mRNA level 24 h after electroporation mediated DNA transfer.

bioscience, biotechnology, and biochemistry

Transient Expression of Goat Growth Hormone Gene in Poplar (<i>Populus alba</i>L.) Protoplasts: A Quick Method for Detection of Foreign Gene Expression in mRNA Level

Transient Expression of Goat Growth Hormone Gene in Poplar (Populus albaL.) Protoplasts: A Quick Method for Detection of Foreign Gene Expression in mRNA Level