Orientation of Photosynthetic Reaction Center Reconstituted in Neutral and Charged Liposomes
Author(s) -
Masayuki Hara,
Takao Ueno,
Takaaki Fujii,
Qing Yang,
Yasuo Asada,
Jun Miyake
Publication year - 1997
Publication title -
bioscience biotechnology and biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.509
H-Index - 116
eISSN - 1347-6947
pISSN - 0916-8451
DOI - 10.1271/bbb.61.1577
Subject(s) - rhodobacter sphaeroides , photosynthetic reaction centre , liposome , photosynthesis , chemistry , rhodobacter , biophysics , orientation (vector space) , center (category theory) , photochemistry , crystallography , biochemistry , biology , geometry , mathematics , mutant , gene
The photosynthetic reaction center from the photosynthetic bacterium Rhodobacter sphaeroides was reconstituted into neutral, positively charged, or negatively charged liposomes. About 70% of photosynthetic reaction centers were reconstituted in the proteoliposomes exposing their H-subunit outside with positively charged lipids while only 30-40% of them were in the same topological orientation with neutral or negatively charged lipids.
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