Expression of a Functional Sphingomyelinase ofPseudomonassp. TK4 in Mammalian Cells | Zendy

Yasuhiro Horibata | Zendy; Noriyuki Sueyoshi | Zendy; Makoto Ito | Zendy

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Expression of a Functional Sphingomyelinase ofPseudomonassp. TK4 in Mammalian Cells

Author(s) -

Yasuhiro Horibata,

Noriyuki Sueyoshi,

Makoto Ito

Publication year - 2007

Publication title -

bioscience biotechnology and biochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.509

H-Index - 116

eISSN - 1347-6947

pISSN - 0916-8451

DOI - 10.1271/bbb.60544

Subject(s) - acid sphingomyelinase , sphingomyelin , ceramide , sphingolipid , pseudomonas , microbiology and biotechnology , biochemistry , sphingomyelin phosphodiesterase , biology , chemistry , bacteria , membrane , genetics , apoptosis

We report here the expression of a bacterial sphingomyelinase in mammalian cells as a functionally active form. A chimeric Pseudomonas sphingomyelinase fused with the lysosomal sorting motif of lysosomal acid phosphatase was sorted to lysosomes in mammalian cells. As expected, the chimeric SMase hydrolyzed sphingomyelin in vivo to produce ceramide, part of which was converted to glucosylceramide.

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