Augmentation of Antigen-Specific Antibody Production and IL-10 Generation with a Fraction from Rooibos (Aspalathus linearis) Tea | Zendy

Kenji Ichiyama | Zendy; Akihiro Tai | Zendy; Itaru Yamamoto | Zendy

Open Access

Augmentation of Antigen-Specific Antibody Production and IL-10 Generation with a Fraction from Rooibos (Aspalathus linearis) Tea

Author(s) -

Kenji Ichiyama,

Akihiro Tai,

Itaru Yamamoto

Publication year - 2007

Publication title -

bioscience biotechnology and biochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.509

H-Index - 116

eISSN - 1347-6947

pISSN - 0916-8451

DOI - 10.1271/bbb.60536

Subject(s) - chemistry , ovalbumin , splenocyte , fraction (chemistry) , chromatography , antibody , aqueous solution , boiling , methanol , elution , immunoglobulin e , in vitro , antigen , biochemistry , immunology , biology , organic chemistry

Rooibos tea was extracted with boiling water. The aqueous extract was chromatographed in a Diaion HP20 column eluted stepwise with water, 25%, 50% and 75% (v/v) aqueous methanol, and 100% methanol. The water eluate (fraction A) showed an augmenting effect on anti-ovalbumin (anti-OVA) immunoglobulin M (IgM) production in OVA-stimulated murine splenocytes in vitro. Fraction A also showed a strong augmenting effect on interleukin-10 generation in murine splenocytes. Furthermore, continuous ingestion of fraction A was found to increase the anti-OVA IgM level in the sera of OVA-immunized mice.

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