Specific Detection of Soybean Residues in Processed Foods by the Polymerase Chain Reaction
Author(s) -
Hirohito YAMAKAWA,
Hiroshi Akiyama,
Yumi ENDO,
Kiyoko MIYATAKE,
Kozue Sakata,
Shinobu SAKAI,
Tatsuya Moriyama,
Atsuo Urisu,
Tamio Maitani
Publication year - 2007
Publication title -
bioscience biotechnology and biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.509
H-Index - 116
eISSN - 1347-6947
pISSN - 0916-8451
DOI - 10.1271/bbb.60485
Subject(s) - polymerase chain reaction , primer (cosmetics) , food science , chemistry , glycine , chromatography , gene , biology , microbiology and biotechnology , biochemistry , organic chemistry , amino acid
A sensitive qualitative detection method for soybeans in foods by using the polymerase chain reaction (PCR) was developed. For specific detection of soybeans with high specificity, the primer pair of Gym 81/Gym 82 was designed on the gene encoding the Glycine max repetitive sequence. The trace amount of soybeans in commercial food products could be qualitatively detected by this method.
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