Enzymatic Preparation of Metabolic Intermediates, 3-Dehydroquinate and 3-Dehydroshikimate, in the Shikimate Pathway | Zendy

Osao Adachi | Zendy; Yoshitaka Ano | Zendy; Hirohide Toyama | Zendy; Kazunobu Matsushita | Zendy

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Enzymatic Preparation of Metabolic Intermediates, 3-Dehydroquinate and 3-Dehydroshikimate, in the Shikimate Pathway

Author(s) -

Osao Adachi,

Yoshitaka Ano,

Hirohide Toyama,

Kazunobu Matsushita

Publication year - 2006

Publication title -

bioscience biotechnology and biochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.509

H-Index - 116

eISSN - 1347-6947

pISSN - 0916-8451

DOI - 10.1271/bbb.60414

Subject(s) - shikimate pathway , dehydratase , shikimic acid , enzyme , biochemistry , chemistry , metabolic pathway , product inhibition , biosynthesis , acetic acid , non competitive inhibition

A method for enzymatic preparation of 3-dehydroquinate and 3-dehydroshikimate in the shikimate pathway was established by controlling the enzyme activity of 3-dehydroquinate dehydratase. When quinate was incubated with the membrane fraction of acetic acid bacteria at pH 5.0, 3-dehydroquinate was formed as the predominant product. 3-Dehydroshikimate was the sole product when incubated at pH 8.0. Mutual separation of the metabolic intermediates was also exemplified.

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