Molecular Cloning of CYP76B9, a Cytochrome P450 fromPetunia hybrida, Catalyzing the ω-Hydroxylation of Capric Acid and Lauric Acid | Zendy

Hiromasa Imaishi | Zendy; Mariana Petkova | Zendy

Open Access

Molecular Cloning of CYP76B9, a Cytochrome P450 fromPetunia hybrida, Catalyzing the ω-Hydroxylation of Capric Acid and Lauric Acid

Author(s) -

Hiromasa Imaishi,

Mariana Petkova

Publication year - 2007

Publication title -

bioscience biotechnology and biochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.509

H-Index - 116

eISSN - 1347-6947

pISSN - 0916-8451

DOI - 10.1271/bbb.60396

Subject(s) - capric acid , lauric acid , decanoic acid , biochemistry , fatty acid , cytochrome p450 , yeast , biology , complementary dna , chemistry , chromatography , enzyme , gene

A cDNA encoding a cytochrome P450 (CYP76B9) was isolated from Petunia hybrida. Northern blot analysis revealed preferential expression of the gene in flowers and leaves. The recombinant yeast microsomes expressing CYP76B9 was allowed to react with capric acid and lauric acid as substrates. One major metabolite was produced from each fatty acid after incubation with yeast microsomes expressing CYP76B9. The metabolites were identified by gas chromatography-mass spectrometry (GC-MS) as omega-hydroxy capric acid and omega-hydroxy lauric acid. The kinetic parameters of the reactions were Km=9.4 microM and Vmax=13.6 mol min(-1) per mol of P450 for capric acid, and Km=5.7 microM and Vmax=19.1 mol min(-1) per mol of P450 for lauric acid. We found that the omega-hydroxy metabolites of capric acid and lauric acid can affect the plant growth of Arabidopsis thaliana. Plants grown in the presence of omega-hydroxy fatty acids exhibited shorter root length than control plants with the corresponding non-hydroxylated fatty acids.

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