Flavonoid Glycosides Extracted from Hop (Humulus lupulusL.) as Inhibitors of Chemical Mediator Release from Human Basophilic KU812 Cells | Zendy

Shuichi Segawa | Zendy; Kazuhisa Yasui | Zendy; Yoshihiro TAKATA | Zendy; Toshio Kurihara | Zendy; Hirotaka Kaneda | Zendy; Junji Watari | Zendy

Open Access

Flavonoid Glycosides Extracted from Hop (Humulus lupulusL.) as Inhibitors of Chemical Mediator Release from Human Basophilic KU812 Cells

Author(s) -

Shuichi Segawa,

Kazuhisa Yasui,

Yoshihiro TAKATA,

Toshio Kurihara,

Hirotaka Kaneda,

Junji Watari

Publication year - 2006

Publication title -

bioscience biotechnology and biochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.509

H-Index - 116

eISSN - 1347-6947

pISSN - 0916-8451

DOI - 10.1271/bbb.60384

Subject(s) - chemistry , humulus lupulus , quercetin , histamine , glycoside , flavonoid , chromatography , biochemistry , food science , pharmacology , organic chemistry , biology , pepper , antioxidant

The antiallergic properties of hop water extract (HWE) were studied by evaluating histamine release from human basophilic KU812 cells induced by calcium ionophore A23187. HWE significantly inhibited histamine release, but boiling water extract and chloroform-methanol extract did not show any inhibitory effect on it. A 50% methanol-eluted fraction separated from HWE by XAD-4 column chromatography (MFH) had a strong inhibitory effect as compared with HWE. Quercetin glycosides and kaempherol glycosides were identified in MFH, of which quercetin glycosides contributed to the inhibition of histamine release. Most quercetin in HWE existed in glycoside form and its quercetin content, obtained by acid hydrolysis, was about 200 mug/g. HWE and MFH significantly inhibited protein kinase C, which plays a pivotal role in the degranulation of chemical mediators. These results indicate that HWE can inhibit type-I allergic reactions.

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