Molecular Cloning ofStreptomycesGenes Encoding Vanillate Demethylase | Zendy

Motohiro Nishimura | Zendy; Daisuke Ishiyama | Zendy; Julian Davies | Zendy

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Molecular Cloning ofStreptomycesGenes Encoding Vanillate Demethylase

Author(s) -

Motohiro Nishimura,

Daisuke Ishiyama,

Julian Davies

Publication year - 2006

Publication title -

bioscience biotechnology and biochemistry

Language(s) - Uncategorized

Resource type - Journals

SCImago Journal Rank - 0.509

H-Index - 116

eISSN - 1347-6947

pISSN - 0916-8451

DOI - 10.1271/bbb.60180

Subject(s) - demethylase , gene , vanillic acid , streptomyces , molecular cloning , biology , protein subunit , ferredoxin , cloning (programming) , genetics , demethylation , microbiology and biotechnology , biochemistry , enzyme , peptide sequence , gene expression , dna methylation , epigenetics , bacteria , food science , computer science , programming language

The vanillate demethylase genes from Streptomyces sp. NL15-2K were cloned and sequenced. The vanA and vanB gene homologs, which encode the terminal oxygenase subunit (VanA) and the ferredoxin-type reductase subunit (VanB) of the enzyme respectively, were found in the sequenced 7.5-kb DNA region. Expression of the vanAB genes in Streptomyces lividans 1326 resulted in in vivo demethylation of veratric acid to vanillic acid.

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