A Rapid Method for Protein Extraction from Fission Yeast | Zendy

Yuzy Matsuo | Zendy; Kazuhide Asakawa | Zendy; Takashi Toda | Zendy; Satoshi Katayama | Zendy

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A Rapid Method for Protein Extraction from Fission Yeast

Author(s) -

Yuzy Matsuo,

Kazuhide Asakawa,

Takashi Toda,

Satoshi Katayama

Publication year - 2006

Publication title -

bioscience biotechnology and biochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.509

H-Index - 116

eISSN - 1347-6947

pISSN - 0916-8451

DOI - 10.1271/bbb.60087

Subject(s) - yeast , fission , extraction (chemistry) , protein purification , blot , chemistry , chromatography , biochemistry , physics , nuclear physics , gene , neutron

Researchers working with fission yeast conduct protein extraction widely and frequently, but this includes the handling of glass beads, and hence is laborious and cumbersome, especially when dealing with a large number of samples. Here we describe a rapid and reliable method for preparing protein extract from fission yeast, one which is applicable to routine western blotting.

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