Cloning of Full-Length cDNA of Teleost Corticotropin-Releasing Hormone Precursor by Improved Inverse PCR | Zendy

Hisato Kuniyoshi | Zendy; Yukio Fukui | Zendy; Yoichi Sakai | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Open Access

Cloning of Full-Length cDNA of Teleost Corticotropin-Releasing Hormone Precursor by Improved Inverse PCR

Author(s) -

Hisato Kuniyoshi,

Yukio Fukui,

Yoichi Sakai

Publication year - 2006

Publication title -

bioscience biotechnology and biochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.509

H-Index - 116

eISSN - 1347-6947

pISSN - 0916-8451

DOI - 10.1271/bbb.60067

Subject(s) - complementary dna , rapid amplification of cdna ends , cloning (programming) , microbiology and biotechnology , biology , computational biology , molecular cloning , gene , computer science , genetics , programming language

We designed a new inverse PCR protocol combined with switching mechanism at 5' end of RNA transcript (SMART) technology, and applied it to the cloning of teleost corticotropin-releasing hormone precursor cDNA. Due to the advantages of both techniques, this method can efficiently amplify the complete 5'- and 3'-ends of cDNA in a single reaction, and might prove to be an alternative to the conventional rapid amplification of cDNA ends (RACE) approaches.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research