Active Transport Activities of Free B-6 Vitamers in Various Yeast Strains
Author(s) -
Toshiharu Yagi,
Yeongmu Kim,
Yasuhisa Hiraoka,
Atsuyo Tanouchi,
Tomokazu Yamamoto,
Shinpei Yamamoto
Publication year - 1996
Publication title -
bioscience biotechnology and biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.509
H-Index - 116
eISSN - 1347-6947
pISSN - 0916-8451
DOI - 10.1271/bbb.60.893
Subject(s) - yeast , pyridoxamine , biochemistry , pyridoxine , chemistry , vitamin , vitamin b , biology , vitamin b6
Active transport activities of free B-6 vitamers in 35 strains of 8 genera of yeast were measured by isocratic reverse-phase HPLC. Many but not all strains transported pyridoxamine and/or pyridoxine. The active transport activities in some yeast strains tested were completely inhibited by amiloride (0.5 mM). In contrast to cells so far studied, yeast cells showed a novel character in metabolism of accumulated B-6 vitamers: the phosphorylation of the free B-6 vitamers was regulated at a low level. There was no apparent correlation between the presence of the active transport activity in yeasts and the requirement of vitamin B-6 for their growth.
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