Duration of Treatment of Carrot Hypocotyl Explants with 2,4-Dichlorophenoxyacetic Acid for Direct Somatic Embryogenesis
Author(s) -
Yoshihiko Tokuji,
Hiroshi Masuda
Publication year - 1996
Publication title -
bioscience biotechnology and biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.509
H-Index - 116
eISSN - 1347-6947
pISSN - 0916-8451
DOI - 10.1271/bbb.60.891
Subject(s) - hypocotyl , somatic embryogenesis , explant culture , embryogenesis , botany , biology , 2,4 dichlorophenoxyacetic acid , chemistry , horticulture , embryo , microbiology and biotechnology , biochemistry , in vitro
The relationship between the concentration of 2,4-dichlorophe- noxyacetic acid (2,4-D) and the duration of treatment with 2,4-D on direct somatic embryogenesis from carrot hypocotyl explants was examined. Elevated concentrations of 2,4-D (up to 100 mg/ liter) reduced the duration of pretreatment required for successful embryogenesis.
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